Biochemistry for Medical Professionals

Biochemistry for Medical Professionals

Tsugikazu Komoda

Language: English

Pages: 112

ISBN: 0128019182

Format: PDF / Kindle (mobi) / ePub

Biochemistry for Medical Professionals

contains pivotal advances in the biochemistry field and provides a resource for professionals across medicine, dentistry, pharmaceutical sciences and health professions who need a concise, topical biochemistry reference.

Relevant, well-illustrated coverage begins with the composition of the human body and then goes into the technical detail of the metabolism of the human body and biochemistry of internal organs before featuring a biotechnology study inclusive of numerous methods and applications.

The work is written at a consistently high level, with technical notes added to aid comprehension for complex topics.

  • Illustrates disease involvement in metabolic maps
  • Contains coverage of cutting-edge technology, including iPS, HPLC and HPLC-MS, and FACS method
  • Provides in-depth technical detail as well as conceptual frameworks of biochemistry and experimental design in the context of the human organism
  • Includes a biotechnology study, featuring application of basic biochemistry principles

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Acetyl-CoA is also obtained from b-oxidation of fatty acids and metabolism of amino acids. Under anaerobic conditions, pyruvate is converted into lactate by lactate dehydrogenase. In patients with acidosis, such as in hyperventilation or diabetes, lactate is converted to pyruvate. Therefore, the reaction of lactate dehydrogenase is reversible and depends on the levels of oxygen in vivo. Metabolic Pathways in the Human Body 33 Metabolism of the Tricarboxylic Acid Cycle and Electron Transfer

blood and 40 mmHg in arterial blood. Since carbon dioxide is extracted from 100 ml of blood under resting conditions, if cardiac output is 5000 ml/minute, the rate of carbon dioxide excretion will be 200 ml/minute. Most of the carbon dioxide produced by metabolism is stored in plasma as bicarbonate (HCO3−) after gas exchange in the lungs. These bicarbonate ions play an important role in buffering the pH of blood. For example, the reaction lactic acid {ReversReact} H+ + lactate ion− takes place,

chromatography. It does not usually contain water. It is suitable for the separation of compounds, such as acid anhydrides, which can be easily hydrolyzed. Reversephase chromatography is a variation of normal-phase chromatography. It uses not only the silica gel stationary phase of conventional normal-phase chromatography but also the stationary phase that introduces the surface of polar compounds. Therefore, chemicals are separable by the mobile phase containing water that could not be used in a

Fundamental View of Relative Quantitation Theoretically, since amplification products double in number every cycle, the difference in Ct value implies exponentiation of two, but PCR does not follow this theory. Therefore, the amplification efficiency should be compared for accurate quantification, even if the products are amplified using the same primers. The cycle reflecting this amplification efficiency is an analytical curve. The analytical curves are amplified with a series of samples of

(pDON-5) were used. G3T-hi cells (2 × 106) were Biotechnological Study 91 seeded on a 6 cm collagen-coated dish. On the next day, evaluation of the pE-ampho vector (1 mg) and pGP vector (2 mg), which are components of Retrovirus Packaging Kit Ampho, and the iPSCs, was carried out using TransIT-293 (10 ml). Method The iPSC colony obtained by the RetroNectin method was taken up and cloned. The copy number of a provirus inserted into a genome was calculated by real-time PCR using the Provirus

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